CHARACTERISATION OF WHOLE PLANT OF APAMARGA (ACHYRANTHUS ASPERA) VIS-A-VIS PHYSICO-CHEMICAL PROPERTIES, HPTLC AND POWDER MICROSCOPY
DOI:
https://doi.org/10.70066/jahm.v5i4.342Keywords:
Apamarga, Achyranthus Aspera Analytical parameters, Physico-chemical, Powder microscopy, HPTLCAbstract
Context: Physico-chemical analysis of drug Apamarga (Achyranthus Aspera) to know the genuine drug.
Aims: Evaluation of Physico-chemical parameters and to develop analytical profile of drug Apamarga
(Achyranthus Aspera). Methods and Material: As the Apamarga is grown everywhere like a weed and easily
available also possess various medicinal properties was collected from specific source in partially dried form
and dried completely, made in to powder form and various analysis like Organoleptic characters, Physicochemical,
HPTLC (High performance thin layer chromatography) & powder microscopy carried out. Results:
The colour of powder was greenish brown, with bitter taste, the results obtained from analytical study shows
that it contain 11.65% of moisture even after complete drying and pH 6.5 shows mild acidic in nature, and Ash
value 9.75%, HPTLC photo documentation and Rf, (Retention factor) at 254nm, 366nm and 620nm after post
Derivatization different peaks and Rf values at different nanometers was recorded and Rf value – 0.63 (purple)
after post Derivatization – suggested the presence of oleanolic acid. Whereas powder microscopy explains
about the presence of Pith parenchyma, Parenchyma with starch, Sclereids, seed testa etc. Conclusion: As the
Apamarga is grown everywhere very commonly and possess various medicinal properties also can be used in
different dosage forms and useful in wide range of therapeutic applications, hence the authentic Apamarga can
be inferred by above said organoleptic characters and analytical parameters.
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