Quantification of total phenols and pharmacognostical evaluation including HPTLC finger printing for different Kashaya kalpana of Cuminum Cyminum - An analytical study
DOI:
https://doi.org/10.70066/jahm.v13i6.2045Keywords:
Decoction, Kwath, Hima, Phanta, Jeeraka, Phenols, Rf Values, Cumin, HPTLC, TLCAbstract
Background: Cuminum cyminumL., known as Jeeraka is used as daily spice and flavouring agent in many cousins. Ayurveda enlists this drug “Jeeraka” as deepana and pachana drug. These seeds have stimulating, analgesic, and carminative properties. The present study provides well-designed and highly appropriate pre-clinical trial of three different aqueous extracts namely kwatha (decoction), hima (maceration), phanta (hot infusion) which differ in their procedures which produce variation in bioactive constituents of cumin. This study aims to quantify the total phenols in different preparations of Cuminum cyminum and further characterise the different formulation (Kwatha, hima and phanta) of Cuminum Cyminum by HPTLC analysis.
Methods: This was an analytical study conducted in AYUSH accredited, Central Research Laboratory. The three drug sample of kwatha, hima and phanta kalpana of jeeraka required for the study were prepared as per the Ayurveda Pharmacopoeia and tested for its taxonomic authentication, phytochemical analysis, total phenolic content and pharmacognostical evaluation using HPTLC finger printing.
Results: In the present study the Drug Sample A-kwatha kalpana had lesser phenolic content of 1621.87 ±3.79 µg GAE/ml of sample when compared to the Drug Sample C- Hima having 1892.17 ±1.05µg GAE/ml and Drug Sample B-Phanta having 2350.73 ±3.79µg GAE/ml. Further the HPTLC for the 3 samples, in terms of maximum AUC (area under curve) and number of Rf (retention factors) values, the sample prepared by the Phanta (hot infusion method), and Kwatha (decoction) appears to be the best.
Conclusion: The drug jeeraka is used in various forms. This study could identify the best form of aqueous extract of cumin are phanta and kwatha with better phytochemical properties and potential benefits.
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