Different diagnostic modes used to check stability study of Gandhakadi Yoga (used in Thalassemia major as adjuvant treatment) for the assessment of baseline microbial profile.

Abstract

Thalassemia major is a malignant type of genetic disorder and iron overload is the main complication of the disease which results due to frequent blood transfusions. Gandhakadi Yoga is one of the Herbo-mineral formulation mentioned which was used in clinical trial to treat Thalassemia major. Objective: In the present study, stability with respect to its microbial profile in different climate condition of Gandhakadi Yoga Vati was carried out. Methods: Gandhakadi Yoga Vati was stored in air tight plastic bag of 50 gm each with numbers given to each bag in room temperature, dark and dry place during different climatic condition. The drug was studied at different intervals for a period of 13 months from June 2022 to June 2023 for the assessment of mycological findings and presence of microorganisms by Wet mount preparation and Gram stain test respectively. Results and Conclusions: At the end of study, no contamination found in prepared drug at minimum humidity of 38% with 22°C temperature and at maximum humidity of 91% with 29°C temperature.